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microarray hybridization chamber kit  (Agilent technologies)


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    Structured Review

    Agilent technologies microarray hybridization chamber kit
    Microarray Hybridization Chamber Kit, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/microarray+hybridization+chamber/pmc10127108-156-15-19
    Average 90 stars, based on 1 article reviews
    microarray hybridization chamber kit - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Microarray:

    Article Title: Integrated Microarray-Based Data Analysis of miRNA Expression Profiles: Identification of Novel Biomarkers of Cisplatin-Resistance in Testicular Germ Cell Tumours.
    Article Snippet: .. The loaded slide, placed in the Agilent Microarray Hybridization Chamber (G2534), was incubated for 20 h at 55 ◦C/20 rpm in the Agilent hybridization oven (G2545A). .. After washing with the Gene Expression Wash Buffers 1 and 2 (5188-5327), the washed and dried slide was scanned in the Agilent SureScan Dx Microarray Scanner (Santa Clara, CA, USA) with a resolution of 3 μm.

    Article Title: Integrated Microarray-Based Data Analysis of miRNA Expression Profiles: Identification of Novel Biomarkers of Cisplatin-Resistance in Testicular Germ Cell Tumours
    Article Snippet: .. The loaded slide, placed in the Agilent Microarray Hybridization Chamber (G2534), was incubated for 20 h at 55 °C/20 rpm in the Agilent hybridization oven (G2545A). .. After washing with the Gene Expression Wash Buffers 1 and 2 (5188-5327), the washed and dried slide was scanned in the Agilent SureScan Dx Microarray Scanner (Santa Clara, CA, USA) with a resolution of 3 μm.

    Article Title: Diverse Effect of Two Cytokinins, Kinetin and Benzyladenine, on Plant Development, Biotic Stress Tolerance, and Gene Expression
    Article Snippet: The hybridization protocol was processed through a hybridization oven (Agilent Technologies Inc., Santa Clara, CA, USA). .. Then, a gasket slide (Agilent Technologies Inc., Santa Clara, CA, USA) was stuck on the microarray slide and then put it in to Agilent Microarray Hybridization Chamber (Agilent Technologies Inc., Santa Clara, CA, USA). .. The hybridization master mix solution was prepared with components from a Gene Expression Hybridization Kit (Agilent Technologies Inc., Santa Clara, CA, USA) and prepared according to the Agilent protocol.

    Article Title: Genomic and transcriptomic insights into the thermo-regulated biosynthesis of validamycin in Streptomyces hygroscopicus 5008
    Article Snippet: The Cy3-labeled cRNA was purified by QIAGEN RNeasy Mini Kit. .. Hybridization was performed in an Agilent Microarray Hybridization Chamber (Agilent G2545A) for 17 h at 65°C. .. After hybridization, the slides were washed in Gene Expression Wash Buffer (Agilent), and the microarrays were scanned (Agilent G2565BA).

    Article Title: Identification of factors required for meristem function in Arabidopsis using a novel next generation sequencing fast forward genetics approach
    Article Snippet: .. Elution was performed using 800 μl of elution buffer (10 mM Tris pH 8.0) in an Agilent Microarray Hybridization Chamber at 95°C for 30 minutes. ..

    Article Title: DNM3OS Enhances the Apoptosis and Senescence of Spermatogonia Associated with Nonobstructive Azoospermia by Providing miR-214-5p and Decreasing E2F2 Expression
    Article Snippet: Subsequently, an aliquot of total RNA (100 ng) derived from testicular samples was labeled using a Quick Amp Labeling Kit (Agilent Technologies, Santa Clara, CA, USA). .. Next, each sample was hybridized with an Agilent Gene Expression Hybridization Kit on a Microarray Hybridization Chamber. .. After washing, the hybrid signal value was scanned with an Agilent G2565BA microarray scanner (Agilent Technologies).

    Article Title: Development of a novel multiplex DNA microarray for Fusarium graminearum and analysis of azole fungicide responses
    Article Snippet: .. Hybridization was set up in a Microarray Hybridization Chamber (Agilent) and was incubated in a hybridization oven (Agilent) at 65°C and 10 rpm for 17 h. After performing the suggested washing steps, slides were scanned by a microarray scanner (Agilent) at 5 μm resolution. .. The microarray scan data were analyzed by Agilent's Feature Extraction software, which estimates the background level and gives a feature-specific background cut-off level per chip.

    Article Title: microRNA expression profiling as supportive diagnostic and therapy prediction tool in chronic myeloid leukemia.
    Article Snippet: .. The loaded slide, placed in the Agilent Microarray Hybridization Chamber (G2534), was incubated for 20 hrs at 55°C/20 rpm in the Agilent hybridization oven (G2545A). .. After washing with the Gene Expression Wash Buffers 1 and 2 (5188-5327), the washed and dried slide was scanned in the Innoscan 900 microarray scanner (Innopsys Inc., Carbonne, France) with the resolution of 3 micrometers.

    Hybridization:

    Article Title: Integrated Microarray-Based Data Analysis of miRNA Expression Profiles: Identification of Novel Biomarkers of Cisplatin-Resistance in Testicular Germ Cell Tumours.
    Article Snippet: .. The loaded slide, placed in the Agilent Microarray Hybridization Chamber (G2534), was incubated for 20 h at 55 ◦C/20 rpm in the Agilent hybridization oven (G2545A). .. After washing with the Gene Expression Wash Buffers 1 and 2 (5188-5327), the washed and dried slide was scanned in the Agilent SureScan Dx Microarray Scanner (Santa Clara, CA, USA) with a resolution of 3 μm.

    Article Title: Integrated Microarray-Based Data Analysis of miRNA Expression Profiles: Identification of Novel Biomarkers of Cisplatin-Resistance in Testicular Germ Cell Tumours
    Article Snippet: .. The loaded slide, placed in the Agilent Microarray Hybridization Chamber (G2534), was incubated for 20 h at 55 °C/20 rpm in the Agilent hybridization oven (G2545A). .. After washing with the Gene Expression Wash Buffers 1 and 2 (5188-5327), the washed and dried slide was scanned in the Agilent SureScan Dx Microarray Scanner (Santa Clara, CA, USA) with a resolution of 3 μm.

    Article Title: Diverse Effect of Two Cytokinins, Kinetin and Benzyladenine, on Plant Development, Biotic Stress Tolerance, and Gene Expression
    Article Snippet: The hybridization protocol was processed through a hybridization oven (Agilent Technologies Inc., Santa Clara, CA, USA). .. Then, a gasket slide (Agilent Technologies Inc., Santa Clara, CA, USA) was stuck on the microarray slide and then put it in to Agilent Microarray Hybridization Chamber (Agilent Technologies Inc., Santa Clara, CA, USA). .. The hybridization master mix solution was prepared with components from a Gene Expression Hybridization Kit (Agilent Technologies Inc., Santa Clara, CA, USA) and prepared according to the Agilent protocol.

    Article Title: Genomic and transcriptomic insights into the thermo-regulated biosynthesis of validamycin in Streptomyces hygroscopicus 5008
    Article Snippet: The Cy3-labeled cRNA was purified by QIAGEN RNeasy Mini Kit. .. Hybridization was performed in an Agilent Microarray Hybridization Chamber (Agilent G2545A) for 17 h at 65°C. .. After hybridization, the slides were washed in Gene Expression Wash Buffer (Agilent), and the microarrays were scanned (Agilent G2565BA).

    Article Title: Identification of factors required for meristem function in Arabidopsis using a novel next generation sequencing fast forward genetics approach
    Article Snippet: .. Elution was performed using 800 μl of elution buffer (10 mM Tris pH 8.0) in an Agilent Microarray Hybridization Chamber at 95°C for 30 minutes. ..

    Article Title: DNM3OS Enhances the Apoptosis and Senescence of Spermatogonia Associated with Nonobstructive Azoospermia by Providing miR-214-5p and Decreasing E2F2 Expression
    Article Snippet: Subsequently, an aliquot of total RNA (100 ng) derived from testicular samples was labeled using a Quick Amp Labeling Kit (Agilent Technologies, Santa Clara, CA, USA). .. Next, each sample was hybridized with an Agilent Gene Expression Hybridization Kit on a Microarray Hybridization Chamber. .. After washing, the hybrid signal value was scanned with an Agilent G2565BA microarray scanner (Agilent Technologies).

    Article Title: Development of a novel multiplex DNA microarray for Fusarium graminearum and analysis of azole fungicide responses
    Article Snippet: .. Hybridization was set up in a Microarray Hybridization Chamber (Agilent) and was incubated in a hybridization oven (Agilent) at 65°C and 10 rpm for 17 h. After performing the suggested washing steps, slides were scanned by a microarray scanner (Agilent) at 5 μm resolution. .. The microarray scan data were analyzed by Agilent's Feature Extraction software, which estimates the background level and gives a feature-specific background cut-off level per chip.

    Article Title: microRNA expression profiling as supportive diagnostic and therapy prediction tool in chronic myeloid leukemia.
    Article Snippet: .. The loaded slide, placed in the Agilent Microarray Hybridization Chamber (G2534), was incubated for 20 hrs at 55°C/20 rpm in the Agilent hybridization oven (G2545A). .. After washing with the Gene Expression Wash Buffers 1 and 2 (5188-5327), the washed and dried slide was scanned in the Innoscan 900 microarray scanner (Innopsys Inc., Carbonne, France) with the resolution of 3 micrometers.

    Incubation:

    Article Title: Integrated Microarray-Based Data Analysis of miRNA Expression Profiles: Identification of Novel Biomarkers of Cisplatin-Resistance in Testicular Germ Cell Tumours.
    Article Snippet: .. The loaded slide, placed in the Agilent Microarray Hybridization Chamber (G2534), was incubated for 20 h at 55 ◦C/20 rpm in the Agilent hybridization oven (G2545A). .. After washing with the Gene Expression Wash Buffers 1 and 2 (5188-5327), the washed and dried slide was scanned in the Agilent SureScan Dx Microarray Scanner (Santa Clara, CA, USA) with a resolution of 3 μm.

    Article Title: Integrated Microarray-Based Data Analysis of miRNA Expression Profiles: Identification of Novel Biomarkers of Cisplatin-Resistance in Testicular Germ Cell Tumours
    Article Snippet: .. The loaded slide, placed in the Agilent Microarray Hybridization Chamber (G2534), was incubated for 20 h at 55 °C/20 rpm in the Agilent hybridization oven (G2545A). .. After washing with the Gene Expression Wash Buffers 1 and 2 (5188-5327), the washed and dried slide was scanned in the Agilent SureScan Dx Microarray Scanner (Santa Clara, CA, USA) with a resolution of 3 μm.

    Article Title: Development of a novel multiplex DNA microarray for Fusarium graminearum and analysis of azole fungicide responses
    Article Snippet: .. Hybridization was set up in a Microarray Hybridization Chamber (Agilent) and was incubated in a hybridization oven (Agilent) at 65°C and 10 rpm for 17 h. After performing the suggested washing steps, slides were scanned by a microarray scanner (Agilent) at 5 μm resolution. .. The microarray scan data were analyzed by Agilent's Feature Extraction software, which estimates the background level and gives a feature-specific background cut-off level per chip.

    Article Title: microRNA expression profiling as supportive diagnostic and therapy prediction tool in chronic myeloid leukemia.
    Article Snippet: .. The loaded slide, placed in the Agilent Microarray Hybridization Chamber (G2534), was incubated for 20 hrs at 55°C/20 rpm in the Agilent hybridization oven (G2545A). .. After washing with the Gene Expression Wash Buffers 1 and 2 (5188-5327), the washed and dried slide was scanned in the Innoscan 900 microarray scanner (Innopsys Inc., Carbonne, France) with the resolution of 3 micrometers.

    Gene Expression:

    Article Title: DNM3OS Enhances the Apoptosis and Senescence of Spermatogonia Associated with Nonobstructive Azoospermia by Providing miR-214-5p and Decreasing E2F2 Expression
    Article Snippet: Subsequently, an aliquot of total RNA (100 ng) derived from testicular samples was labeled using a Quick Amp Labeling Kit (Agilent Technologies, Santa Clara, CA, USA). .. Next, each sample was hybridized with an Agilent Gene Expression Hybridization Kit on a Microarray Hybridization Chamber. .. After washing, the hybrid signal value was scanned with an Agilent G2565BA microarray scanner (Agilent Technologies).



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    Mature miR- 221 is overexpressed in AML. 22 blast-enriched AML samples and 9 healthy controls (3 CD34+, 3 BM, 3 PB), all of which had also been included in the <t>microarray</t> experiments, were subjected to Taqman qRT-PCR to measure mature miR-221 (A) or miR-222 (B) . Expression values were calculated according to the ΔΔCt method , with RNU6B as internal reference and PB from healthy donor A as calibrator. *, p < 0.05; n.s., not significant (miR-222, p = 0.064).
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    Mature miR- 221 is overexpressed in AML. 22 blast-enriched AML samples and 9 healthy controls (3 CD34+, 3 BM, 3 PB), all of which had also been included in the <t>microarray</t> experiments, were subjected to Taqman qRT-PCR to measure mature miR-221 (A) or miR-222 (B) . Expression values were calculated according to the ΔΔCt method , with RNU6B as internal reference and PB from healthy donor A as calibrator. *, p < 0.05; n.s., not significant (miR-222, p = 0.064).
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    Agilent technologies microarray hybridization chambers
    A) Gene expression levels of Angiotensin II receptors (Agtr1 and Agtr2) were evaluated by qPCR. B) Technical validation of oligonucleotide <t>microarray</t> data by qPCR of Ang II-regulated genes. The expression of the genes Agtrap, Map2k4, Bcar1, Lamb1, Prkca, Hbegf, Aqp2, Vegfa, Ctgf and Nlrp3 was assessed to confirm gene expression changes identified by oligonucleotide microarray analysis. The gene Gapdh was used as an internal control. The comparison is made between average log2 expression values derived from microarray experiments and arbitrary units obtained from qPCR assays.
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    Image Search Results


    Mature miR- 221 is overexpressed in AML. 22 blast-enriched AML samples and 9 healthy controls (3 CD34+, 3 BM, 3 PB), all of which had also been included in the microarray experiments, were subjected to Taqman qRT-PCR to measure mature miR-221 (A) or miR-222 (B) . Expression values were calculated according to the ΔΔCt method , with RNU6B as internal reference and PB from healthy donor A as calibrator. *, p < 0.05; n.s., not significant (miR-222, p = 0.064).

    Journal: BMC Cancer

    Article Title: Overexpression of primary microRNA 221/222 in acute myeloid leukemia

    doi: 10.1186/1471-2407-13-364

    Figure Lengend Snippet: Mature miR- 221 is overexpressed in AML. 22 blast-enriched AML samples and 9 healthy controls (3 CD34+, 3 BM, 3 PB), all of which had also been included in the microarray experiments, were subjected to Taqman qRT-PCR to measure mature miR-221 (A) or miR-222 (B) . Expression values were calculated according to the ΔΔCt method , with RNU6B as internal reference and PB from healthy donor A as calibrator. *, p < 0.05; n.s., not significant (miR-222, p = 0.064).

    Article Snippet: Arrays were hybridized overnight at 60°C in microarray hybridization chambers (Corning, Corning, NY, USA).

    Techniques: Microarray, Quantitative RT-PCR, Expressing

    A) Gene expression levels of Angiotensin II receptors (Agtr1 and Agtr2) were evaluated by qPCR. B) Technical validation of oligonucleotide microarray data by qPCR of Ang II-regulated genes. The expression of the genes Agtrap, Map2k4, Bcar1, Lamb1, Prkca, Hbegf, Aqp2, Vegfa, Ctgf and Nlrp3 was assessed to confirm gene expression changes identified by oligonucleotide microarray analysis. The gene Gapdh was used as an internal control. The comparison is made between average log2 expression values derived from microarray experiments and arbitrary units obtained from qPCR assays.

    Journal: PLoS ONE

    Article Title: Transcriptional Network Analysis Reveals that AT1 and AT2 Angiotensin II Receptors Are Both Involved in the Regulation of Genes Essential for Glioma Progression

    doi: 10.1371/journal.pone.0110934

    Figure Lengend Snippet: A) Gene expression levels of Angiotensin II receptors (Agtr1 and Agtr2) were evaluated by qPCR. B) Technical validation of oligonucleotide microarray data by qPCR of Ang II-regulated genes. The expression of the genes Agtrap, Map2k4, Bcar1, Lamb1, Prkca, Hbegf, Aqp2, Vegfa, Ctgf and Nlrp3 was assessed to confirm gene expression changes identified by oligonucleotide microarray analysis. The gene Gapdh was used as an internal control. The comparison is made between average log2 expression values derived from microarray experiments and arbitrary units obtained from qPCR assays.

    Article Snippet: Arrays were incubated at 65°C for 17 h in Agilent's microarray hybridization chambers and subsequently washed according to Agilent's one-color microarray-based gene expression analysis protocol (Version 5.7, March 2008).

    Techniques: Expressing, Microarray, Derivative Assay